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magnetic bead labeling  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic bead labeling
    Magnetic Bead Labeling, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+magnetic+beads/CD11b+MicroBeads%2C+human+and+mouse/pm42468771-93-9-13
    Average 96 stars, based on 512 article reviews
    magnetic bead labeling - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Dual Role of Ninjurin-1 in Myeloid Cell Adhesion and Inflammation in Relapse-Remitting EAE
    Article Snippet: .. Splenocytes were removed from naïve C57BL/6 mice and enriched with CD11b magnetic beads from Miltenyi Biotec. .. Cells were then stained with Ninjurin-1, CD45, CD11b, CD3, B220, Ly6G, and Live Dead antibodies ( Fig. S4 ) and sorted into two populations, CD45 + CD11b + B220 - CD3 - Ly6G - Ninjurin-1 + (Ninjurin-1 + ) or CD45 + CD11b + B220 - CD3 - Ly6G - Ninjurin-1 - (Ninjurin-1 - ) using a MA900 (Sony).

    Article Title: Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice.
    Article Snippet: To isolate CD11b+ cells from all other cells in joint-associated tissue, single-cell suspensions were generated as previously described and mononuclear cells were isolated by density gradient centrifugation using Lympholyte-M Cell Separation Media (CL5031; Cedarlane). .. Purified cells were labelled with CD11b magnetic beads (130-049- 601; Miltenyi Biotec) and flowed through a MACS column to capture labelled cells. ..

    Article Title: Dual role of Ninjurin-1 in myeloid cell adhesion and inflammation in relapse-remitting EAE.
    Article Snippet: .. Splenocytes were removed from naïve C57BL/6 mice and enriched with CD11b magnetic beads from Miltenyi Biotec. .. Cells were then stained with Ninjurin-1, CD45, CD11b, CD3, B220, Ly6G, and Live Dead antibodies (Supplementary Figure 4) and sorted into two populations, CD45+CD11b+B220-CD3-Ly6GNinjurin-1+ (Ninjurin-1+) or CD45+CD11b+B220-CD3-Ly6GNinjurin-1- (Ninjurin-1-) using a MA900 (Sony).

    Article Title: Microglial reactivity and neuroinflammation-driven changes in motivational behaviors are regulated by Orai1 calcium channels
    Article Snippet: .. The pellet was resuspended in 100 μl of magnetic-activated cell sorting (MACS) buffer (130–091-221, Miltenyi Biotec) and incubated with 20 μl of CD11b magnetic beads (130–093-636, Miltenyi Biotec) for 15 min at 4°C. ..

    Article Title: Successful dendritic cell vaccines require lasting in-situ TNFa secretion to license antitumor CD8 + T cell cytotoxicity
    Article Snippet: .. For prophylactic vaccination, classical splenic cDCs and MoDCs cultured from blood monocytes were isolated on CD11c and CD11b magnetic beads (Miltenyi Biotec), respectively from C57Bl/6 mice. .. Cells were then activated overnight with 1 μg/mL of poly I:C or LPS (InvivoGen) and pulsed with 5 μM of MHC-I-restricted peptides gp100 (EGSRNQDWL) and TRP1 (TAPDNLGYM) (Syntezza Bioscience) for an additional one hours.

    Article Title: Dual role of Ninjurin-1 in myeloid cell adhesion and inflammation in relapse-remitting EAE
    Article Snippet: .. Splenocytes were removed from naïve C57BL/6 mice and enriched with CD11b magnetic beads from Miltenyi Biotec. .. Cells were then stained with Ninjurin-1, CD45, CD11b, CD3, B220, Ly6G, and Live Dead antibodies ( ) and sorted into two populations, CD45 + CD11b + B220 - CD3 - Ly6G - Ninjurin-1 + (Ninjurin-1 + ) or CD45 + CD11b + B220 - CD3 - Ly6G - Ninjurin-1 - (Ninjurin-1 - ) using a MA900 (Sony).

    Article Title: Neuroinflammatory stress preferentially impacts synaptic MAPK signaling and mitochondria in excitatory neurons
    Article Snippet: .. Forty μL CD11b + magnetic beads (Miltenyi Biotec, 130–093–636) were added to the cell suspension and incubated protected from light for 15 min. ..

    Article Title: Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice
    Article Snippet: To isolate CD11b + cells from all other cells in joint-associated tissue, single-cell suspensions were generated as previously described and mononuclear cells were isolated by density gradient centrifugation using Lympholyte-M Cell Separation Media (CL5031; Cedarlane). .. Purified cells were labelled with CD11b magnetic beads (130-049-601; Miltenyi Biotec) and flowed through a MACS column to capture labelled cells. ..

    Purification:

    Article Title: Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice.
    Article Snippet: To isolate CD11b+ cells from all other cells in joint-associated tissue, single-cell suspensions were generated as previously described and mononuclear cells were isolated by density gradient centrifugation using Lympholyte-M Cell Separation Media (CL5031; Cedarlane). .. Purified cells were labelled with CD11b magnetic beads (130-049- 601; Miltenyi Biotec) and flowed through a MACS column to capture labelled cells. ..

    Article Title: Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice
    Article Snippet: To isolate CD11b + cells from all other cells in joint-associated tissue, single-cell suspensions were generated as previously described and mononuclear cells were isolated by density gradient centrifugation using Lympholyte-M Cell Separation Media (CL5031; Cedarlane). .. Purified cells were labelled with CD11b magnetic beads (130-049-601; Miltenyi Biotec) and flowed through a MACS column to capture labelled cells. ..

    Magnetic Cell Separation:

    Article Title: Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice.
    Article Snippet: To isolate CD11b+ cells from all other cells in joint-associated tissue, single-cell suspensions were generated as previously described and mononuclear cells were isolated by density gradient centrifugation using Lympholyte-M Cell Separation Media (CL5031; Cedarlane). .. Purified cells were labelled with CD11b magnetic beads (130-049- 601; Miltenyi Biotec) and flowed through a MACS column to capture labelled cells. ..

    Article Title: Microglial reactivity and neuroinflammation-driven changes in motivational behaviors are regulated by Orai1 calcium channels
    Article Snippet: .. The pellet was resuspended in 100 μl of magnetic-activated cell sorting (MACS) buffer (130–091-221, Miltenyi Biotec) and incubated with 20 μl of CD11b magnetic beads (130–093-636, Miltenyi Biotec) for 15 min at 4°C. ..

    Article Title: Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice
    Article Snippet: To isolate CD11b + cells from all other cells in joint-associated tissue, single-cell suspensions were generated as previously described and mononuclear cells were isolated by density gradient centrifugation using Lympholyte-M Cell Separation Media (CL5031; Cedarlane). .. Purified cells were labelled with CD11b magnetic beads (130-049-601; Miltenyi Biotec) and flowed through a MACS column to capture labelled cells. ..

    FACS:

    Article Title: Microglial reactivity and neuroinflammation-driven changes in motivational behaviors are regulated by Orai1 calcium channels
    Article Snippet: .. The pellet was resuspended in 100 μl of magnetic-activated cell sorting (MACS) buffer (130–091-221, Miltenyi Biotec) and incubated with 20 μl of CD11b magnetic beads (130–093-636, Miltenyi Biotec) for 15 min at 4°C. ..

    Incubation:

    Article Title: Microglial reactivity and neuroinflammation-driven changes in motivational behaviors are regulated by Orai1 calcium channels
    Article Snippet: .. The pellet was resuspended in 100 μl of magnetic-activated cell sorting (MACS) buffer (130–091-221, Miltenyi Biotec) and incubated with 20 μl of CD11b magnetic beads (130–093-636, Miltenyi Biotec) for 15 min at 4°C. ..

    Article Title: Neuroinflammatory stress preferentially impacts synaptic MAPK signaling and mitochondria in excitatory neurons
    Article Snippet: .. Forty μL CD11b + magnetic beads (Miltenyi Biotec, 130–093–636) were added to the cell suspension and incubated protected from light for 15 min. ..

    Cell Culture:

    Article Title: Successful dendritic cell vaccines require lasting in-situ TNFa secretion to license antitumor CD8 + T cell cytotoxicity
    Article Snippet: .. For prophylactic vaccination, classical splenic cDCs and MoDCs cultured from blood monocytes were isolated on CD11c and CD11b magnetic beads (Miltenyi Biotec), respectively from C57Bl/6 mice. .. Cells were then activated overnight with 1 μg/mL of poly I:C or LPS (InvivoGen) and pulsed with 5 μM of MHC-I-restricted peptides gp100 (EGSRNQDWL) and TRP1 (TAPDNLGYM) (Syntezza Bioscience) for an additional one hours.

    Isolation:

    Article Title: Successful dendritic cell vaccines require lasting in-situ TNFa secretion to license antitumor CD8 + T cell cytotoxicity
    Article Snippet: .. For prophylactic vaccination, classical splenic cDCs and MoDCs cultured from blood monocytes were isolated on CD11c and CD11b magnetic beads (Miltenyi Biotec), respectively from C57Bl/6 mice. .. Cells were then activated overnight with 1 μg/mL of poly I:C or LPS (InvivoGen) and pulsed with 5 μM of MHC-I-restricted peptides gp100 (EGSRNQDWL) and TRP1 (TAPDNLGYM) (Syntezza Bioscience) for an additional one hours.

    Suspension:

    Article Title: Neuroinflammatory stress preferentially impacts synaptic MAPK signaling and mitochondria in excitatory neurons
    Article Snippet: .. Forty μL CD11b + magnetic beads (Miltenyi Biotec, 130–093–636) were added to the cell suspension and incubated protected from light for 15 min. ..



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    Decreased MPO + , <t>CD11b</t> + , and MHC class II + neutrophils in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–G) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. Non-infected BALB/c mice ( n = 10/2 feet per mouse) were given 0.9% NaCl saline. Cells were isolated from the footpads of all infected and non-infected mice at days 3 and 14 PI. Samples were analyzed by flow cytometry for MPO + , CD11b + , and MHC class II + neutrophils. A representative flow cytometry dot plot of MPO + , CD11b + , and MHC class II + neutrophils on day 3 is presented in . The percentage of MPO + , CD11b + , and MHC class II + cells in the different experimental groups is shown in column graphs (A, B, C, D, E, and F), while the absolute number of neutrophils obtained on day 3 PI is indicated (G). In all experiments, infected and non-infected BALB/c mice served as positive and negative controls, respectively. Results are presented as mean (±SEM) and are representative of two independent experiments. One-way ANOVA followed by Bonferroni’s post hoc test was used to analyze the data ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, non-significant ( p > 0.05).
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    Image Search Results


    Decreased MPO + , CD11b + , and MHC class II + neutrophils in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–G) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. Non-infected BALB/c mice ( n = 10/2 feet per mouse) were given 0.9% NaCl saline. Cells were isolated from the footpads of all infected and non-infected mice at days 3 and 14 PI. Samples were analyzed by flow cytometry for MPO + , CD11b + , and MHC class II + neutrophils. A representative flow cytometry dot plot of MPO + , CD11b + , and MHC class II + neutrophils on day 3 is presented in . The percentage of MPO + , CD11b + , and MHC class II + cells in the different experimental groups is shown in column graphs (A, B, C, D, E, and F), while the absolute number of neutrophils obtained on day 3 PI is indicated (G). In all experiments, infected and non-infected BALB/c mice served as positive and negative controls, respectively. Results are presented as mean (±SEM) and are representative of two independent experiments. One-way ANOVA followed by Bonferroni’s post hoc test was used to analyze the data ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, non-significant ( p > 0.05).

    Journal: iScience

    Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis

    doi: 10.1016/j.isci.2026.115090

    Figure Lengend Snippet: Decreased MPO + , CD11b + , and MHC class II + neutrophils in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–G) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. Non-infected BALB/c mice ( n = 10/2 feet per mouse) were given 0.9% NaCl saline. Cells were isolated from the footpads of all infected and non-infected mice at days 3 and 14 PI. Samples were analyzed by flow cytometry for MPO + , CD11b + , and MHC class II + neutrophils. A representative flow cytometry dot plot of MPO + , CD11b + , and MHC class II + neutrophils on day 3 is presented in . The percentage of MPO + , CD11b + , and MHC class II + cells in the different experimental groups is shown in column graphs (A, B, C, D, E, and F), while the absolute number of neutrophils obtained on day 3 PI is indicated (G). In all experiments, infected and non-infected BALB/c mice served as positive and negative controls, respectively. Results are presented as mean (±SEM) and are representative of two independent experiments. One-way ANOVA followed by Bonferroni’s post hoc test was used to analyze the data ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, non-significant ( p > 0.05).

    Article Snippet: CD11b + monocytes were isolated and purified from female mouse bone marrow cells using CD11b MicroBeads UltraPure mouse magnetic bead (Miltenyi Biotec) according to the manufacturer’s protocol.

    Techniques: Infection, Saline, Isolation, Flow Cytometry

    Increased CD38 + macrophages and CD8α + dendritic cells in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–D) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. One week and two weeks post-infection, cells from the infected foot of each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) , and non-infected BALB/c mice ( n = 10/2 feet per mouse) were harvested and counted for macrophages (A and B) and dendritic cells (C and D) by flow cytometry. (E–H) Two weeks post-infection, the infected foot from each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) ( n = 5 per group), and non-infected BALB/c mice ( n = 10/2 feet per mouse) were examined for CD206 + and CD38 + macrophages (E and F), as well as CD11b + and CD8α + dendritic cells (G and H) by flow cytometry. Representative flow cytometry dot plots showing the analysis of CD206 + , CD38 + macrophages and CD11b + , CD8α + dendritic cells and a dot plot of each sample in all the experimental groups performed on day 14 PI are presented in . Results are presented as mean ± SEM and are representative of two independent experiments. Data analysis was done using one-way ANOVA followed by Bonferroni’s post hoc test ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and “ns” indicates non-significant.

    Journal: iScience

    Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis

    doi: 10.1016/j.isci.2026.115090

    Figure Lengend Snippet: Increased CD38 + macrophages and CD8α + dendritic cells in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–D) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. One week and two weeks post-infection, cells from the infected foot of each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) , and non-infected BALB/c mice ( n = 10/2 feet per mouse) were harvested and counted for macrophages (A and B) and dendritic cells (C and D) by flow cytometry. (E–H) Two weeks post-infection, the infected foot from each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) ( n = 5 per group), and non-infected BALB/c mice ( n = 10/2 feet per mouse) were examined for CD206 + and CD38 + macrophages (E and F), as well as CD11b + and CD8α + dendritic cells (G and H) by flow cytometry. Representative flow cytometry dot plots showing the analysis of CD206 + , CD38 + macrophages and CD11b + , CD8α + dendritic cells and a dot plot of each sample in all the experimental groups performed on day 14 PI are presented in . Results are presented as mean ± SEM and are representative of two independent experiments. Data analysis was done using one-way ANOVA followed by Bonferroni’s post hoc test ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and “ns” indicates non-significant.

    Article Snippet: CD11b + monocytes were isolated and purified from female mouse bone marrow cells using CD11b MicroBeads UltraPure mouse magnetic bead (Miltenyi Biotec) according to the manufacturer’s protocol.

    Techniques: Infection, Flow Cytometry